Thanks to the support of the Integrated Research Training Group of CRC1211, I had the invaluable opportunity to spend four weeks gaining practical research experience in Germany and Sweden. This cross-border academic journey deepened my understanding of molecular techniques and species identification, directly contributing to the success of my research project titled: "Assessment of Biodiversity of Free-Living Soil and Freshwater Nematodes in the Namib Desert."
Week 1: Molecular Techniques in Cologne, Germany
From May 12th to May 16th 2025, I was based at the University of Cologne, where I focused on molecular biology techniques essential for DNA analysis. The week was intensive and practical, involving:
- DNA quantification and gel electrophoresis
- Assessing the quality of extracted DNA
- Preparing extracted DNA for sequencing using the Oxford Nanopore platform
We followed detailed protocols to prepare the library media and load it into the sequencing machine. The sequencing process ran throughout the weekend, generating valuable data that would form the basis of our bioinformatics training in the following weeks.
Weeks 2–3: Bioinformatics and Data Analysis
During the second and third weeks, we shifted focus to bioinformatics — learning how to process and analyze sequencing data using Bash and the command line interface. This phase of the training was both challenging and rewarding, as we learned how to convert raw sequencing data into readable and interpretable formats. The skills I gained during this time are directly applicable to the computational analysis I will conduct for my own research.
Week 4: Morphological Identification in Sweden
The final week took us to Sweden, where we had the privilege of learning from an expert in nematode taxonomy, Dr Oleksander Holovachov at the Swedish Museum of Natural history in Stockholm. The focus here was on morphological identification of nematodes — a vital complement to the molecular tools we learned in Germany.
Each day followed a structured format:
Morning sessions were devoted to theoretical lessons on different nematode groups — including plant-parasitic, predatory, aquatic nematodes as well as soil nematodes.
Afternoons were spent analyzing prepared slides under the microscope and using identification keys to determine the Family group. This hands-on approach reinforced my understanding of nematode biodiversity and gave me the dual perspective of both molecular and morphological analysis — a rare and valuable combination.
The entire experience was eye-opening. It provided me with the practical tools and knowledge I need to successfully carry out my research project. The exposure to advanced laboratory protocols, data analysis software, and expert mentorship is something I would not have had access to otherwise. I'm incredibly grateful for the opportunity to engage in such a diverse and impactful learning experience. It has not only enriched my academic skills but also broadened my perspective on international collaboration in scientific research.
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Text: Socha Geirises
